By M. L. Thakur (auth.), Max R. Hardeman PhD, Yves Najean MD (eds.)
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Extra info for Blood cells in nuclear medicine, part I: Cell kinetics and bio-distribution
18 : 1014, 1977. 2. Schmidt KG, Rasmussen JW, Scand. J. Haanat. 23:97, 1979. 3. Weiblen BJ, Forstran L, McCullough J, J. Lab. elin. Med. 94:246, 1979. 4. Hawker ru, Hall CE, Thranb. Haenost. 46:433, 1981. 5. R5vekamp MH,Thesis, University of Amsterdam, 1982. 6. Hardanan MR, In: Disease Evaluation and Patient Assessment in Rheumatoid Arthritis, Stafleu's Scientific Publ. Co. Alphen ad Rijn, Brussels, 1979. 7. Hardanan MR, Brit. J. Radiol. 53:927, 1980. 8. Murphy S, Gardner PH, New Engl. J. Med. 280:1094, 1969.
C) Prostacyclin (PGI 2 ). s. 36 at 4°C). 1 ml aliquots and kept at -30°C for at least 6 months. 2. Radioactive products. lllIndium oxinate (Ref. IN-15P) and 3H-5-hydroxytryptamine (Ref. TRK 223) were obtained from Amersham and 125I-albumine (Ref. SARI 125 A2 3. Platelet aggregating~gents ) from CIS, France. (6). ADP (Ref. A-0127) and collagen (C-9879 was from Sigma. Bovine thrombin (Ref. 4010) was from Roche. C. Preparation of 111 In-labelled washed human platelets. The method used to prepare washed labelled human platelets for survival studies is a modification of the method developed by Mustard and collaborators (5).
Using 750 WI volumes of blood pre- anG post-passage through the columns, the percentage radioactivity retained was calculated by direct measurement of radioactivity (Searle Automatic Gamma Systems). Following centrifugation of the same samples (Thrombofuge, Coulter Electronics Ltd) to produce platelet-rich plasma, a platelet count was made (Coulter Model FBI) and the percentage 47 of platelets retained calculated from the numerical data. C. Measurement of free Indium in ex vivo blood-samples. 5 ml blood-samples were taken in clean venepuncture into standard haematology tubes (Sterilin KE/5) containing potassium EDTA.
Blood cells in nuclear medicine, part I: Cell kinetics and bio-distribution by M. L. Thakur (auth.), Max R. Hardeman PhD, Yves Najean MD (eds.)