By Mitchelson, Cheng
ISBN-10: 0896037657
ISBN-13: 9780896037656
ISBN-10: 1592591167
ISBN-13: 9781592591169
An exceptional panel of hands-on specialists and builders of CE apparatus describe in step by step type their most sensible state-of-the-art tools for the detection and research of DNA mutations and adjustments, starting from certain DNA loci to complete genomes of organisms. This moment quantity of the set, functional purposes of Capillary Electrophoresis, covers thoughts for high-throughput research of DNA fragments utilizing SNP detection, mutation detection, DNA sequencing tools, and DNA-ligand interactions. a number of chapters additionally speak about CE research at increased temperatures and using micro-CE and array-CE units. The better half quantity, advent to the Capillar Electrophoresis of Nucliec Acids, deals with ease reproducible tools for the research of small oligonucleotides and transformed nucleotides, and time-tested suggestion on instrumentation, sign detection, the capillary atmosphere, and the combination of mass spectrometry with CE. entire and up to date, the paired volumes of Capillary Electrophoresis of Nucleic Acids provide an authoritative advisor with easy accessibility to speedy, flexible, trustworthy, and strong applied sciences for all these simple and scientific investigators reading DNA edition this present day.
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Extra resources for Capillary Electrophoresis of Nucleic Acids Volume 2 Practical Applications of Capillary Electrophoresis (Methods in Molecular Biology Vol 163)
Example text
4. As a further measure of reproducibility using these fast run conditions, we determined the ratio of both peak height and peak area for two adjacent peaks (Table 2B). 10. 6. Minisequencing Product Analysis 1. , sickle cell anemia) and as genetic markers in linkage analysis studies. A variety of techniques are currently employed to examine specific nucleotide compositions at defined positions in DNA (15). One of these techniques, minisequencing (16), or single nucleotide extension, employs template directed primer extension by a single fluorescently labeled nucleotide to interrogate individual loci.
Adapted with permission from ref. 29. Copyright (1997) American Chemical Society. 36 Klepárník, Mueller, and Foret Fig. 11. (A) Diagram of an electrophoresis chip with integrated electrochemical detection. (B) Separation obtained on this chip of a Salmonella PCR product (shaded) along with 500 pg/mL of ΦX174/HaeIII restriction digest. Adapted with permission from ref. 30. Copyright (1998) American Chemical Society. analysis speed, the current technology also provides sensitive LIF detection for all critical applications.
This allows the accurate sizing of DNA fragments that differ in size by 4 bp. 7% precision. 4. As a further measure of reproducibility using these fast run conditions, we determined the ratio of both peak height and peak area for two adjacent peaks (Table 2B). 10. 6. Minisequencing Product Analysis 1. , sickle cell anemia) and as genetic markers in linkage analysis studies. A variety of techniques are currently employed to examine specific nucleotide compositions at defined positions in DNA (15).
Capillary Electrophoresis of Nucleic Acids Volume 2 Practical Applications of Capillary Electrophoresis (Methods in Molecular Biology Vol 163) by Mitchelson, Cheng
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