Read e-book online Culture of Human Stem Cells (Culture of Specialized Cells) PDF

By R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach

ISBN-10: 0470052465

ISBN-13: 9780470052464

This book collects the simplest and state of the art tools and protocols for deriving and culturing human embryonic and grownup stem cells—in one convenient resource.
This groundbreaking book follows the culture of prior books within the tradition of specialised Cells Series—each equipment and protocols bankruptcy is laid out precisely just like the subsequent, with stepwise protocols, preceded through particular requisites for that protocol, and a concise dialogue of equipment illustrated via information. The editors describe a constrained variety of consultant suggestions throughout a large spectrum of stem cells from embryonic, baby, and grownup tissue, yielding an all-encompassing and flexible consultant to the sector of stem telephone biology and culture.
The ebook encompasses a accomplished checklist of providers for all gear utilized in the protocols offered, with web content on hand in an appendix. also, there's a bankruptcy on quality controls, and different chapters masking criminal and moral matters, cryopreservation, and feeder layer culture. This textual content is a one-stop source for all researchers, medical scientists, academics, and scholars serious about this significant zone of analysis.

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Additional info for Culture of Human Stem Cells (Culture of Specialized Cells)

Example text

1. Outline of hES Cell Differentiation. . . . . . . . . . . . . . . . . . . . . . . . 2. Embryoid Body Formation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14. Generation of Embryoid Bodies from hES Cells . . . . . . . . . . . 9. Sources of Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . References . .

10. Thawing hES Cells Cryopreserved by Vitrification . . . . . . . . . . 7. Characterization of hES Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1. Immunocytochemical Characterization of hES Cells . . . . . . . . . . . . . 11. Characterization of hES Cells by Fluorescence Immunocytochemistry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Et al. (1977). Inter- and intraspecies contamination of human breast tumor cell lines HBC and BrCa5 and other cell cultures. Science 195:1343–1344. R. (1981). Cross-contamination of cells in culture. Science 212:446–452. , Olson, R. E. (1977). Enzyme polymorphisms as genetic signatures in human cell cultures. Science 195:1345–1348. K. (1979). Measurement of growth and viability of cells in culture. Methods Enzymol. 58:141–152. M. (2006). Characterization of a new NIH registered variant human embryonic stem cell line BG01V: A tool for human embryonic stem cell research.

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Culture of Human Stem Cells (Culture of Specialized Cells) by R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach


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