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Extra info for Current Protocols in Molecular Biology - Chapter 11 Immunology

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Contains excellent introductions to experimental techniques for working with E. coli and λ-derived phages. , ed. 1981. The Operon. Y. Contributed by Elisabeth A. 5 Plasmids are self-replicating, extrachromosomal DNA molecules found in virtually all bacterial species. In nature, plasmids occur in exuberant profusion, varying in structure, size, mode of replication, number of copies per bacterial cell, ability to propagate in different bacteria, transferability between bacterial species, and perhaps most important, in the traits they carry.

1983. Construction of improved M13 vectors using oligonucleotide-directed mutagenesis. Gene 26:101-106. A. 1987. Cosmid vectors for rapid genomic walking, restriction mapping, and gene transfer. Proc. Natl. Acad. Sci. A. 84:2160-2164. R. 1990. Genetic characterization of the stabilizing functions of a region of broad-host-range plasmid RK2. J. Bacteriol. 172:6204-6216. I. 1992. A bacterial system for cloning large human DNA fragments. Proc. Natl. Acad. Sci. A. 89:8794-8797. R. 1996. Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein.

6; Sikorski and Hieter, 1989). These vectors have a backbone derived from pBLUESCRIPT into which the features necessary for replication and maintenance in yeast have been introduced. The members of this series of plasmids differ only in the yeast selectable marker incorporated; pRS303 carries the HIS3 marker that complements a nonreverting his3 chromosomal mutation in specific yeast strains. These plasmids contain an autonomously replicating sequence as well as a centromere sequence, CEN6, that ensures stable maintenance in yeast cells.

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Current Protocols in Molecular Biology - Chapter 11 Immunology


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