Differentiated Cells in Aging Research - download pdf or read online

By Edited by Warren W. Nichols and Donald G. Murphy

ISBN-10: 0123643104

ISBN-13: 9780123643100

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Paradoxically, the aldehyde derivative of isosafrol, piperonal, is Schiff-negative (Lison, 1932). Various aldehydes may be produced from non-aldehydic compounds by suitable techniques. This forms the basis for the histochemical applications of Schiff’s reagent. Deoxyribonucleic acid is hydrolyzed by hydrochloric acid to form aldehyde residues (Feulgen and Rossenbeck, 1924). Acetal phosphatides, known also as plasmalogens, react with mercuric chloride to form higher aldehydes or plasmals (Feulgen and Voit, 1924).

Both of these suggested reactions are shown on page 22; it should be kept in mind that the second and third steps of the hydrolytic reaction scheme are unlikely to occur. Wertheim (1922) claimed that his Schiff’s reagent was more sensitive after having been boiled for 1 minute, and he noted the color changes described above. The phenomena were also studied by Shoesmith et d. (1927). Increased temperature causes an increase in the amount of dye re-formed with formaldehyde (Atkinson, 1952). Since dissociation may occur simultaneously with the aldehyde reaction, heat should be avoided.

Some of the interpretations of Durrschnabel and Weil (1905) regarding the constitution of their reaction products of pararosaniline and H2S03 were disputed by Wieland and Scheuing (1921), who made a thorough study of the problem. Apparently, Durrschnabel and Weil thought they had isolated sulfinic acid derivatives (R-S02H) when actually they had isolated sulfite ester compounds (R-0-S02H) . Wieland and Scheuing also showed that the fuchsin sulfonic acid of Hantzch and Osswald (1900) did not give a color reaction with aldehydes.

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Differentiated Cells in Aging Research by Edited by Warren W. Nichols and Donald G. Murphy


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