By Daryl S. Henderson
Univ. of Dundee, united kingdom. presents specific directions for learning manifold facets of the eukaryotic reaction to genomic damage. Emphasizes tools for studying DNA fix methods in mammalian cells. For researchers. Hardcover, plastic-comb binding additionally on hand.
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Extra info for DNA Repair Protocols: Eukaryotic Systems
6. 4. 7. a-Ray Test the M2 Pools, Step G The purposes of this step are to evaluate carefully the M2 pools for the presence of hypersensitive plants and to eliminate false-positives. The seedlings are grown on agar under sterile conditions and are spaced for maximum visibility (Fig. 1A). 1. 4. 2. Sterilize the seeds (see Note 11): a. Mix 40 mL of bleach with 40 mL of sterile dH2O, and add 80 µL of a 20% Triton X-100 stock solution. b. Place seeds into a sterile container, add bleach solution. c.
Chin, G. , and Hawley, R. S. (1995) The Drosophila meiotic recombination gene mei–9 encodes a homologue of the yeast excision repair protein Rad1. Genetics 141, 619–627. 23. Banga, S. , Yamamoto, A. , Mason, J. M, and Boyd, J. B. (1995) Molecular cloning of mei–41, a gene that influences both somatic and germline chromosome metabolism of Drosophila melanogaster. Mol. Gen. Genet. 246, 148–155. 24. Hari, K. , Sekelsky, J. , McKim, K. , Boyd, J. , and Hawley, R. S. (1995) The mei–41 gene of D. melanogaster is a structural and functional homolog of the human ataxia telangiectasia gene.
Mix and incubate overnight (12–15 h), rotating if possible. 5 N NaOH, and dispose). Rinse three times, and then rinse in 10 mL of dH2O for 2–4 h. 2. Planting and Growth of EMS-Treated Seed, Step B The purpose of this step is to grow to maturity a large population of EMStreated plants in a manner that allows collection of seeds from individual plants. About 200 plants can be grown in a 10 × 20 in. sq. flat. 1. Add water to the soil mixture until the soil is moist, but not saturated. Add the moist soil mixture to a flat.
DNA Repair Protocols: Eukaryotic Systems by Daryl S. Henderson