By H. Tedeschi
In the previous few years, the physique of experimental paintings at the constitution, functionality and meeting procedures of mitochondria has multiplied quickly. not anyone individual can think himself or herself thoroughly accountable for the burgeoning literature with out owning critical omissions or blind spots. within the current monograph i've got tried a serious overview of the literature. i feel that the typical thread of unmarried authorship outweighs the shortcomings of 1 individual featuring many disparate viewpo,ints. it's my desire that the tip product represents a complete and coordinated overview of the subject material to the current date. even supposing the majority of this monograph used to be accomplished by way of October 1974, i've got made a few makes an attempt to replace a number of of the sections at later instances. Albany, N. Y. , November 1975 H. TEDESCHI Contents A. constitution of Mitochondria 1. common association . a) traditional skinny part Electron Microscopy b) damaging Staining recommendations . three c) Freeze-Cleavage . . . . . . . four 2. distinctive association and Inclusions 10 a) Prismatic or extraordinary Cristae 10 b) Filaments and Tubules . . eleven c) Amorphous Inclusions 12 d) Intramitochondrial Granules 12 e) DNA Containing Fibers 14 three. Topography of the Mitochondrial Transducing structures 15 B. The meeting of Mitochondria 18 1. Mitochondrial DNA and Its Genetic position 19 2. Transcription of the Mitochondrial DNA . 28 a) mRNA . . . . . . . . . . . . . . 30 b) The equipment for Transcription and Translation 32 c) Turnover of Mitochondrial RNA 36 three. Protein Synthesis . . . . . . . . 37 a) Mitochondrial Protein Synthesis and Its features 37 b) position of Mitochondrial Protein Synthesis . .
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Additional resources for Mitochondria: Structure, Biogenesis and Transducing Functions
Data from rat liver (BORST and AAIJ 1969) and amphibian eggs (DAWID 1972 a) are in agreement with these findings. 5% of the L strand binds to the RNA in contrast to the virtual 1000/0 binding by the H strand. This complete hybridization was confirmed with both ultracentrifugation and electron microscopy (ALONI and ATTARD! 1972). Experiments carried out with a short pulse give significantly different results (ALON! and ATTARD! 1971 d) from those obtained with prolonged labelling. The level of hybridization to the L strand is sufficiently high to suggest that the two strands hybridize approximately to the same extent.
1972, AAIJ and BORST 1972, REIJNDERS and BORST 1972), Xenopus and HeLa cells (Wu et al. 1972). At least some tRNAs (see later discussion and DAWID 1972 a, Wu et al. 1972, NASS and BUCK 1970, COHEN and RABINOWITZ 1972, CASEY et al. , OJALA and ATTARD! 1974) hybridize to mitochondrial DNA. A good deal is now known about the in vivo transcription of the mitochondrial DNA of HeLa cells (ATTARD! et al. 1970, ALONI and ATTARD! 1971 a, b, c, Wu et al. 1972). The mitochondrial RNA labelled with uridine for a long period (48 hours) was hybridized to the separated Hand L strands of the mitochondria.
Responds to 4 S RNA. The 16 S sector corresponds to the heavy rRNA (about 1570 nucleotides). Then, eight 4 S RNA sections follow (H 3-H 9 and HI). The L strand has three 4 S sequences (Part B of Fig. 8). In rat liver (NASS and BUCK 1970) leucine tRNA and phenylalanine tRNA hybridize to the H strand, tyrosine tRNA and serine tRNA to the L strand. The RNA containing a poly-A sequence and which is thought to correspond to mRNA (HIRSCH et al. 1974, OJALA and ATTARDI 1974) has been calculated to account for a large portion of the HeLa cell DNA not already assigned to tRNA or rRNA (ALONI and ATTARDI 1971 a, b, ROBBERSON et al.
Mitochondria: Structure, Biogenesis and Transducing Functions by H. Tedeschi