Nucleic Acids, Part A, 3rd Edition - download pdf or read online

By Paul D. (Editor); Boyer

ISBN-10: 0080865917

ISBN-13: 9780080865911

ISBN-10: 0121227146

ISBN-13: 9780121227142

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Cairns, Nature (London)224, 1164 (1969). 3. T. Kornberg and M. Gefter, BBHC 40, 1348 (1970). 4. R. Moses and C. C. Richardson, BERC 41, 1557, 1565 (1970). 5. R. Knippers, Nature (London) 228, 1050 (1970). 6. T. Kornberg and M. Gefter, PNAS 68, 761 (1971). 7. M. Gefter, Y. Hirota, T. Kornberg, J. Wechsler, and C. Barnoux, PNAS 68, 3150 (1971). 8. R. Schekman, A. Weiner, and A. Kornberg, Science 186, 987. (1974). 3. DNA POLYMERASE 111 HOLOENZYME 41 I11 was initially detected as a peak of polymerase activity that was eluted at salt concentrations lower than those needed to elute pol I1 from phosphocellulose columns (6).

W. Veomett, BBRC 41, 973 (1970); R. Okazaki, M. Arisawa, and A. Sugino, PNAS 68, 2954 (1971). 35. D. T. Kingsbury, and D. R. Helinski. BBRC 41, 1538 (1970). 30 I. ROBERT LEHMAN can catalyze the concerted 5' + 3' exonucleolytic removal of a DNA segment that includes the dimer, and the 5' + 3' polymerization required to restore the DNA duplex (31). Inasmuch as polA mutants are only moderately sensitive to UV irradiation, it is clear that alternative excision-repair mechanisms must exist that can substitute for pol I when the latter is defective (36).

Nonlethal PolA I2 Mutant Strains bearing thepolA12 mutation show a temperature sensitive repair defect; that is, they are defective in the repair of DNA damage at 43", but not at 30" (39). They are, however, viable at both temperatures. The polAI2 polymerase is extremely thermosensitive. It is also rapidly dena36. P. Hanawalt, A. Burrell, P. Cooper, and W. Masker, In "DNA Synthesis and Its Regulation" (M. Goulian and P. ), p. 774. Benjamin, Menlo Park, 1975. 37. I. R. Lehman, and D. Uyemura, Science 193, %3 (1976).

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Nucleic Acids, Part A, 3rd Edition by Paul D. (Editor); Boyer

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