Erik Dassi (eds.)'s Post-Transcriptional Gene Regulation PDF

By Erik Dassi (eds.)

ISBN-10: 1493930664

ISBN-13: 9781493930661

ISBN-10: 1493930672

ISBN-13: 9781493930678

This quantity goals to supply the latest advances in options for learning gene expression law on the post-transcriptional point. Post-Transcriptional Gene legislation, moment Edition is equipped in six sections describing bioinformatics methods for learning post-transcriptional legislation, numerous expression profiling techniques, protein-RNA interplay and non-coding RNA research concepts, tools for profiling RNA changes, and different concepts comparable to replacement translation initiation or polyadenylation websites choice. Written for the Methods in Molecular Biology sequence, chapters comprise introductions to their respective themes, lists of the required fabrics and reagents, step by step, quite simply reproducible laboratory protocols, and pointers on troubleshooting and averting recognized pitfalls.

Authoritative and useful, Post-Transcriptional Gene rules, moment version is a flexible source to researchers learning post-transcriptional legislation, introducing the newest ideas and offering a complete consultant to their implementation.

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Kearns, and René Maehr Abstract CRISPR/Cas9-based regulation of gene expression provides the scientific community with a new highthroughput tool to dissect the role of genes in molecular processes and cellular functions. Single-guide RNAs allow for recruitment of a nuclease-dead Cas9 protein and transcriptional Cas9-effector fusion proteins to specific genomic loci, thereby modulating gene expression. We describe the application of a CRISPR-Cas9 effector system from Streptococcus pyogenes for transcriptional regulation in mammalian cells resulting in activation or repression of transcription.

RNA (New York, NY) 20:989–993 41. 0: 10 years of RNA families. Nucleic Acids Res 41:D226–D232 42. Muller S, Rycak L, Afonso-Grunz F, Winter P, Zawada AM, Damrath E, Scheider J, Schmah J, Koch I, Kahl G, Rotter B (2014) APADB: a database for alternative polyadenylation and microRNA regulation events. Database 2014:bau076 43. 0: the large and diverse AU-rich transcriptome. Nucleic Acids Res 34:D111–D114 44. Gruber AR, Fallmann J, Kratochvill F, Kovarik P, Hofacker IL (2011) AREsite: a database for the comprehensive investigation of AU-rich elements.

Nucleic Acids Res 38:W360–W367 Jacobs GH, Chen A, Stevens SG, Stockwell PA, Black MA, Tate WP, Brown CM (2009) Transterm: a database to aid the analysis of regulatory sequences in mRNAs. Nucleic Acids Res 37:D72–D76 Agostini F, Zanzoni A, Klus P, Marchese D, Cirillo D, Tartaglia GG (2013) catRAPID omics: a web server for large-scale prediction of protein-RNA interactions. Bioinformatics (Oxford, England) 29:2928–2930 Paz I, Kosti I, Ares M Jr, Cline M, MandelGutfreund Y (2014) RBPmap: a web server for mapping binding sites of RNA-binding proteins.

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Post-Transcriptional Gene Regulation by Erik Dassi (eds.)

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