By Waldo E. Cohn (ed.), Kivie Moldave (ed.)
Meant to be of curiosity to researchers in biochemistry, molecular biology, genetics and mobile biology, this ebook covers such subject matters as ribosome biogenesis in yeast, amplification of DNA sequences in mammalian cells and structural components in RNA.
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Additional info for Progress in Nucleic Acid Research and Molecular Biology, Vol. 41
In Vitro STUDIES Tables I1 and I11 summarize the effects of anticodon base changes on the recognition of tRNAs by cognate and noncognate synthetases in oitro. Significant reductions in the efficiency of aminoacylation of E. coli tRNAs specific for Arg, Gly, Ile, Met, Phe, Thr, Trp, Tyr, and Val result from base changes in the anticodon. The magnitude of the effect varies with the particular synthetase, and may be related to the total number of recognition elements in a given tRNA. MetRS and ThrRS also strongly protect the anticodons of their cognate tRNAs from chemical modification and nuclease attack (85, 86).
5 25 25 29 30 43 44 44 44 51 53 55 57 58 58 60 62 63 64 66 72 81 82 The highly specific selection of tRNA substrates by aminoacyl-tRNA synthetases is an intriguing problem in RNA-protein recognition. Synthetases specific for each of the 20 amino acids encounter a pool of tRNAs in the cell having similar overall structures (1-3). Selection of the appropriate tRNAs for attachment of each amino acid occurs by the formation of RNA-protein contacts unique to each cognate tRNA-synthetase pair.
Cytologically, about 50% of the acinar cells are polyploid within 2 days of treatment. Biochemically, a dramatic induction of the multigene family encoding the PRPs is observed. The expression of PRPs for the parotid and submandibular glands is tissue-specific or, possibly more correctly, cell-specific. PRPs have been identified immunochemically in the trachea (53) and the pancreas but there is no evidence that PRP genes in these tissues respond as in the salivary glands to isoproterenol treatment.
Progress in Nucleic Acid Research and Molecular Biology, Vol. 41 by Waldo E. Cohn (ed.), Kivie Moldave (ed.)