By Adhya S. (ed.), Garges S.
RNA polymerase is molecule very important to gene transcription. besides linked elements, RNA polymerase is a part of the method during which RNA is transcribed to provide a protein.* building and purification of RNA polymerases* DNA microarrays and bacterial gene expression* practical research of transcription components
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31, 1285 (1999). 6 R. H. Doi, in ‘‘The Molecular Biology of the Bacilli’’ (D. A. ), p. 7. Academic Press, New York, 1982. 7 J. L. Wiggs, M. Z. Gilman, and M. J. Chamberlin, Proc. Natl. Acad. Sci. USA 78, 2762 (1981). 8 E. I. Hyde, M. D. Hilton, and H. R. Whiteley, J. Biol. Chem. 261, 16565 (1986). 9 E. C. Achberger and H. R. Whiteley, J. Biol. Chem. 256, 7424 (1981). 10 K. F. Dobinson and G. B. Spiegelman, Biochemistry 26, 8206 (1987). 11 Y. L. Juang and J. D. Helmann, J. Mol. Biol. 239, 1 (1994).
Cells expressing 0 fusion proteins have a reduced ability to grow at tem peratures higher than 37 . 7–9 Thus, strains containing rpoC fusions should be grown at 32 . 2 M NaCl in TGED instead of TGED only. This is done because the NaCl apparently facilitates the 6 D. Yu, H. M. Ellis, E. C. Lee, N. A. Jenkins, N. G. Copeland, and D. L. Court, Proc. Natl. Acad. Sci. USA 97, 5978 (2000). 7 P. A. Levin, I. G. Kurtser, and A. D. Grossman, Proc. Natl. Acad. Sci. USA 96, 9642 (1999). 8 K. R. Siemering, R.
Microbiol. (2003, in press). [2] Purification of Bacillus subtilis RNA Polymerase and Associated Factors By John D. Helmann Introduction Bacterial RNA polymerase (RNAP) has been purified using a wide variety of techniques. In addition to continuing interest in its fundamental enzymatic properties, purified RNAP is used as a tool for the investigation of transcriptional control mechanisms and for the preparation of specific RNA transcripts. This article focuses on techniques developed for the purification of RNAP and its associated factors from the model gram-positive bacterium Bacillus subtilis.
RNA Polymerase and Associated Factors Part C by Adhya S. (ed.), Garges S.
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