By Leen Vijgen PhD, Elien Moës, Els Keyaerts PhD, Sandra Li, Marc Van Ranst MD, PhD (auth.), Dave Cavanagh (eds.)
In 2003, the observe "coronavirus" unfold around the globe, just a little additional than the virus that sparked the panic. In SARS- and different Coronaviruses: Laboratory Protocols, specialist researchers research those devastating viruses via distinctive laboratory protocols. Chapters take care of such matters as detection and discovery of coronaviruses via nucleic acid and antibody-based methods, virus isolation, propagation and titration, virus purification, constitution research by way of electron cryomicroscopy, expression and crystallization of viral proteins, elevating antibodies opposed to viral proteins, manipulation of the coronavirus genome, and outlines of ways to enquire facets of the mobilephone floor for coronavirus receptors. Composed within the hugely winning Methods in Molecular Biology™ sequence layout, each one bankruptcy encompasses a short creation, step by step equipment, an inventory of important fabrics, and a Notes part which stocks tips about troubleshooting and keeping off identified pitfalls.
Comprehensive and state-of-the-art, SARS- and different Coronaviruses: Laboratory Protocols serves as a great advisor for all virologists and particularly for these operating with coronaviruses.
Read or Download SARS- and Other Coronaviruses: Laboratory Protocols PDF
Similar biology books
A set of state-of-the-art suggestions for detecting and quantifying apoptosis, figuring out its biochemistry, and for settling on the genes and proteins that control and hold it out. defined in step by step aspect, those simply reproducible equipment variety from stream cytometry and immunohistochemical tactics to kinase task assays, yeast two-hybrid screening, and the cloning of novel genes by means of differential expression.
With bankruptcy contributions from greater than 30 steel biology specialists, mobile and Molecular Biology of Metals explains the function of key divalent steel ions interested in the molecular and mobile biology of varied aim telephone populations. even though it basically specializes in homeostatic metals, equivalent to nickel, zinc, and chromium, the textual content additionally discusses a couple of environmentally pertinent, poisonous divalent cations, together with mercury, cadmium, and arsenic.
The time period cytoskeleton has turn into firmly demonstrated in contemporary clinical vocabulary. certainly, it's tricky to think that purely ten years in the past, it used to be nearly non-existent. in view that then, the trendy box of analysis at the structural association of the cytoplasm has become probably the most effective and swiftly increasing learn parts in telephone Biology this day.
- The behavior and sensory biology of elasmobranch fishes: an anthology in memory of Donald Richard Nelson
- Pilze: Lebewesen zwischen Pflanze und Tier
- Fluorescent Energy Transfer Nucleic Acid Probes: Designs And Protocols (Methods in Molecular Biology Vol 335)
- Membrane Structure
- Biology of the Vestfold Hills, Antarctica: Proceedings of the symposium, Hobart, August 1984
Additional info for SARS- and Other Coronaviruses: Laboratory Protocols
1999) Phylogenetic analysis of a highly conserved region of the polymerase gene from 11 coronaviruses and development of a consensus polymerase chain reaction assay Virus Res. 60, 181–189. 8. Jonassen, C. , Jonassen, T. , Sveen, T. , and Grinde, B. (2003) Complete genomic sequences of astroviruses from sheep and turkey: comparison with related viruses Virus Res. 91, 195–201. 4 RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot Francesca Anne Culver, Paul Britton, and Dave Cavanagh Abstract Of the many primer combinations that we have investigated for the detection of avian coronaviruses, two have worked better than any of the others: they worked with the largest number of strains/samples of a given coronavirus and the most species of avian coronavirus, and they also produced the most sensitive detection tests.
Agarose Gel Electrophoresis 1. Place a gel-casting tray onto a gel-casting base. Level the base. 2. 75 g of agarose powder. Add it to a 250-ml bottle containing 50 ml 1X TAE buffer. Microwave the bottle with a loosened cap until the gel starts to bubble and become transparent (see Note 10). 2 M 1 U/reaction – – 20 Poon and Peiris Table 3 Conditions for the PCR Reaction Step 1. Heat activation 2. Thermal cycling (45 cycles) Denaturing step Annealing step Extension 3. Final extension 4. Soak Temperature Time ◦ 94 C 10 min 94◦ C 48◦ C 72◦ C 72◦ C 4◦ C 30 sec 30 sec 40 sec 2 min ∞ 3.
Thermocyclers (MJ Research) are used both for RT and PCR. 2. Sequencing and Analysis 1. Agarose (Applied Biosystems) for visualization of PCR products. 2. 3). 3. 68 is used to check for primer hairpin structures and primer dimers and to calculate annealing temperatures for PCR and cycle sequencing. Detection of Coronavirus Genomes Harboring 3’ s2m Sequence 29 4. 1 (Applied Biosystems) is used for sequencing of short (from initial PCR) or long (5 -RACE PCR) PCR products, respectively. 5. Cycle sequencing reaction is performed on an MJ Research thermocycler.
SARS- and Other Coronaviruses: Laboratory Protocols by Leen Vijgen PhD, Elien Moës, Els Keyaerts PhD, Sandra Li, Marc Van Ranst MD, PhD (auth.), Dave Cavanagh (eds.)