By Tony Hugli (Eds.)
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Additional info for Techniques in Protein Chemistry
Since the reaction environment required for production of the PTC-amino acid ester is identical to that necessary to perform the prior Edman step, no stringent acid removal is required. S t e r i c h i n d e r a n c e a s s o c i a t e d with the sensitivity-enhancing alcohols actually promote the desired reaction by disfavoring the recyclization to the PTH derivative. The method gives an improved signal-to-noise ratio since the product's absorbance characteristics differ significantly from Edman by-products.
2 2 7 - 2 3 6 , Academic Press, Orlando, F L . 6. Boguslaw, S. and Summers, D. F . ( 1 9 8 8 ) Anal. Biochem. 168, 4 8 - 5 3 . 7. X u , Q. and Shively, J . E . ( 1 9 8 8 ) Anal. Biochem. 170, 1 9 - 3 0 . 8. Aebersold, R. , Leavitt, J . , Saavedra, R. , Hood, L . E . and K e n t , S. Β. H. ( 1 9 8 7 ) Proc. Natl. Acad. Sci. USA 8 4 , 6 9 7 0 - 6 9 7 4 . 9. 10. Salinovich, O. and Montelaro, R. C. ( 1 9 8 6 ) Anal. Biochem. 156, 3 4 1 347. Hsieh, J . , Lin, F . and Tarn, M. F . ( 1 9 8 8 ) Anal. Biochem.
1985). Nature 318. 659. , and Vale, W. (1986). Proc Natl. Acad. Sci. USA 22, 5849. , and Hood, L. (1983). Methods in Enzymology 21, 227. A. R. (1980). Anal. Biochem. 109. 76. , and Grego, B. (1987). Eur. J. Biochem. 165. 2 1 . H. et al. H. and Spiess, J. (1987). Proc. Natl. Acad. Sci. USA 21, 3628. f 5 DIRECT SEQUENCE ANALYSIS OF RAT LIVER MEMBRANE GLYCOPROTEINS SEPARATED B Y 2D-PAGE David C. Parmelee, Timothy Benjamin, Chien-Hua and Snorri S. Thorgeirsson Niu, National Cancer Institute, NIH, Bldg.
Techniques in Protein Chemistry by Tony Hugli (Eds.)