By G. T. Williams (auth.), Professor Dr. Leslie Hudson (eds.)
African and South American trypanosomiases are impressive good points of medical and veterinary perform of their respective endemic components and, as such, are of substantial monetary significance. Scientifically, even if, their significance ex has a tendency past their scientific importance, because the trypano somes are exciting and simply manipulated versions for the examine of the regulate of gene expression, membrane chemistry, proliferation and differentiation. it really is transparent from the medical press that the speed of improve has "hotted" up in those parts of trypanosome study over the last five years and so a single-topic quantity in the scope of the current sequence appeared well timed. As ever, the ultimate admix ture of overview issues was once a compromise among what used to be acceptable and what used to be on hand - thankfully with the previous in sizeable extra. I should still wish to spotlight omissions, made for en tirely varied purposes. the 1st is a close remedy of the molecular biology of the version floor glycopro teins of the African trypanosomes (in specific Trypano soma brucei and T. equiperdum). This subject has been the topic of numerous studies, for instance, BORST and move (1982)1 and TURNER (1982)2, and so used to be excluded from the current quantity. the second one omission is a evaluation of the first class paintings on genetic recombination from the gang of Dr. Leo Jenni on the Schweizerisches Tropeninsti tut, Basel. This staff has used isoenzyme markers to teach that T.
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Additional info for The Biology of Trypanosomes
Our hypothesis on starting these experiments was that the surface coat was made up of a matrix of VSGs of which some carried the CRD, and were readily solubilised, and some carried the hydrophobic tail and were true intrinsic membrane proteins. This hypothesis was not compatible with the results obtained with the lysates prepared in different detergents. A further surprise came when the amount of VSG per trypanosome was quantitated by radioimmunoassay (RIA). 5% n-octylglucoside were used to inhibit the binding of P25 I]sVSG to the antibody in the construction of a standard curve.
In the light of subsequent developments, the data concerning the expression of hydrophobic precursors can be rationalised, as described in Sect. 5. Experiments to determine the timing of the addition of the CRD, which were not quantitative, strongly suggest that it occurred early within the time frame of the pulse-chase experiments, that is, by about 10 min in a system in which it took 45 min for newly synthesised VSG to reach the plasma membrane. ROVIS and DUBE (1981) suggested that addition of the CRD took place post-translationally, since in their own pulsechase experiments the amount of VSG immunoprecipitable by anti-CRD rose to a maximum after 1 h of the chase.
The interpretation was that a hydrophobic precursor, presumably having the hydrophobic tail, was processed at between 10 and 30 min in a system where it took about 45 min for newly synthesised VSG to reach the plasma membrane. 6 VSG at a point before 10 min, or it is never present in the primary translation product. Why should a hydrophobic precursor be processed to a hydrophilic one? There is a simple post hoc explanation. As will be discussed below, trypanosomes contain a very active enzyme which converts the true, hydrophobic, membrane form of the VSG (mfVSG) into the water-soluble form (sVSG) conventionally isolated.
The Biology of Trypanosomes by G. T. Williams (auth.), Professor Dr. Leslie Hudson (eds.)