Get The Biology of Trypanosomes PDF

By G. T. Williams (auth.), Professor Dr. Leslie Hudson (eds.)

ISBN-10: 3642705383

ISBN-13: 9783642705380

ISBN-10: 3642705405

ISBN-13: 9783642705403

African and South American trypanosomiases are impressive good points of medical and veterinary perform of their respective endemic components and, as such, are of substantial monetary significance. Scientifically, even if, their significance ex­ has a tendency past their scientific importance, because the trypano­ somes are exciting and simply manipulated versions for the examine of the regulate of gene expression, membrane chemistry, proliferation and differentiation. it really is transparent from the medical press that the speed of improve has "hotted" up in those parts of trypanosome study over the last five years and so a single-topic quantity in the scope of the current sequence appeared well timed. As ever, the ultimate admix­ ture of overview issues was once a compromise among what used to be acceptable and what used to be on hand - thankfully with the previous in sizeable extra. I should still wish to spotlight omissions, made for en­ tirely varied purposes. the 1st is a close remedy of the molecular biology of the version floor glycopro­ teins of the African trypanosomes (in specific Trypano­ soma brucei and T. equiperdum). This subject has been the topic of numerous studies, for instance, BORST and move (1982)1 and TURNER (1982)2, and so used to be excluded from the current quantity. the second one omission is a evaluation of the first class paintings on genetic recombination from the gang of Dr. Leo Jenni on the Schweizerisches Tropeninsti­ tut, Basel. This staff has used isoenzyme markers to teach that T.

Show description

Read Online or Download The Biology of Trypanosomes PDF

Best biology books

Apoptosis Methods and Protocols by Demetrius Matassov, Terri Kagan, Julie Leblanc (auth.), Hugh PDF

A set of state-of-the-art suggestions for detecting and quantifying apoptosis, realizing its biochemistry, and for deciding upon the genes and proteins that control and hold it out. defined in step by step aspect, those effectively reproducible tools variety from circulate cytometry and immunohistochemical strategies to kinase job assays, yeast two-hybrid screening, and the cloning of novel genes via differential expression.

Rudolfs K. Zalups, D. James Koropatnick's Cellular and Molecular Biology of Metals PDF

With bankruptcy contributions from greater than 30 steel biology specialists, mobile and Molecular Biology of Metals explains the position of key divalent steel ions inquisitive about the molecular and mobile biology of assorted aim mobilephone populations. even though it essentially makes a speciality of homeostatic metals, similar to nickel, zinc, and chromium, the textual content additionally discusses a number of environmentally pertinent, poisonous divalent cations, together with mercury, cadmium, and arsenic.

Read e-book online The Cytoskeleton: An Introductory Survey PDF

The time period cytoskeleton has develop into firmly verified in modern-day medical vocabulary. certainly, it's tough to think that simply ten years in the past, it was once nearly non-existent. in view that then, the fashionable box of study at the structural association of the cytoplasm has changed into essentially the most effective and speedily increasing learn parts in mobilephone Biology at the present time.

Additional info for The Biology of Trypanosomes

Sample text

Our hypothesis on starting these experiments was that the surface coat was made up of a matrix of VSGs of which some carried the CRD, and were readily solubilised, and some carried the hydrophobic tail and were true intrinsic membrane proteins. This hypothesis was not compatible with the results obtained with the lysates prepared in different detergents. A further surprise came when the amount of VSG per trypanosome was quantitated by radioimmunoassay (RIA). 5% n-octylglucoside were used to inhibit the binding of P25 I]sVSG to the antibody in the construction of a standard curve.

In the light of subsequent developments, the data concerning the expression of hydrophobic precursors can be rationalised, as described in Sect. 5. Experiments to determine the timing of the addition of the CRD, which were not quantitative, strongly suggest that it occurred early within the time frame of the pulse-chase experiments, that is, by about 10 min in a system in which it took 45 min for newly synthesised VSG to reach the plasma membrane. ROVIS and DUBE (1981) suggested that addition of the CRD took place post-translationally, since in their own pulsechase experiments the amount of VSG immunoprecipitable by anti-CRD rose to a maximum after 1 h of the chase.

The interpretation was that a hydrophobic precursor, presumably having the hydrophobic tail, was processed at between 10 and 30 min in a system where it took about 45 min for newly synthesised VSG to reach the plasma membrane. 6 VSG at a point before 10 min, or it is never present in the primary translation product. Why should a hydrophobic precursor be processed to a hydrophilic one? There is a simple post hoc explanation. As will be discussed below, trypanosomes contain a very active enzyme which converts the true, hydrophobic, membrane form of the VSG (mfVSG) into the water-soluble form (sVSG) conventionally isolated.

Download PDF sample

The Biology of Trypanosomes by G. T. Williams (auth.), Professor Dr. Leslie Hudson (eds.)


by David
4.4

Rated 4.70 of 5 – based on 27 votes
This entry was posted in Biology.