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Atkinson, D. M. Small, R. P. Skarjune, E. Oldfield and G. G. Shipley. Biochemistry 20, 5957(1981). 73. -A. Karlsson, B. E. Samuelsson and G. O. Steen. Biochim. Biophys. Ada 306, 317(1973). 74. Y. Barenholz, J. Suurkuusk, D. Mountcastle, T. E. Thompson and R. L. Biltonen. Biochemistry 15, 2441(1976). 75. R. P. Rand and V. A. Parsegian. Ann. Rev. Physiol. 48, 201(1986). 76. J. Coorssen and R. P. Rand. Studia Biophys. Ill, 53(1988). 77. D. M. LeNeveu, R. P. Rand and V. A. Parsegian. Nature 259, 601(1976).

Reprinted with permission from ref. 22. Copyright © 1981 Adenine Press. observation is upheld by the most recent review of hydrogen bonding in crystal structures of nucleic acid components [17] in which about one-third of the observed three-coordinated water molecules have a planar environment. 1. Small hydrates Despite limitations inherent to the method, [6-8] important information can be gathered from analysis of crystal structures of small hydrates. For example, a comparison of crystal structures of adenine derivatives led Rao and Sundaralingam [18] to suggest that protonated adenines have a tendency for C8—H---O hydrogen bonding, apparently through an increase in the polarizability of the C8—H bond (Figure 6).

Gaffney. Proc. Nat. Acad. Sci. USA 77, 5060(1980). 29. L. Finegold and M. A. Singer. Biochim. Biophys. Acta 855, 417(1986). 30. D. G. Cameron and H. H. Mantsch. Biophys. J. 38, 175(1982). 31. H. H. Fuldner. Biochemistry 20, 5707(1981). 32. M. J. Ruocco and G. G. Shipley. Biochim. Biophys. Acta 684, 59(1982). 33. S. F. Bush, H. Levin and I. W. Levin. Chem. Phys. Lipids 27, 101(1980). 34. N. O. Petersen, P. A. Kroon, M. Kainosho and S. I. Chan. Chem. Phys. Lipids 14, 343(1975). 35. H. L. Casal, H.

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