By David Murphy, David A. Carter
Functional tools for the iteration and research of transgenic animals, masking a accomplished diversity of species, from invertebrates to reduce vertebrates to better vertebrates. Chapters research ways that transgenic concepts can be utilized to deal with many difficulties in organic platforms current in-depth descriptions of analytical equipment, and provides step by step strategies that may be simply played on your lab.
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Extra info for Transgenesis Techniques: Principles and Protocols Methods in Molecular Biology Vol 18
1989) Recessive Oncogenes and Tumor Suppression. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 12. , and Coffin, J. , RNA Tumor Viruses, Part 1, Text. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 13. , and Coffin, J. , RNA Tumor Viruses, Part 1, Supplements and Appendixes. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 14. Brshop, J. M. (1984) Cellular oncogenes and retrovrruses. Ann Rev. Blochem. 52,301-354. 15. Nusse, R. and Berns, A. (1988) Cellular oncogene actrvatron by msertron of retrovrral DNA.
Therefore, one can begin to evaluate cooperativity between different genes of From Methods Edited by m Molecular Biology, Vol 18 Transgenesls Techniques Prrmples and Protocols D Murphy and D A Carter Copyrtght 01993 Humana Press Inc , Totowa, NJ 37 HUl diverse cell types within the immune system. These conditions are usually too complex or impossible to reproduce in cultured cells. It is therefore not surprising that a great number of transgenic animals (mostly mice) have been generated to gain new insights mto many of the key immunological questions (24).
14. Robmson, R. , and Mellor, A. L. (1989) A glycophosphohpid anchor is required for Qa-2-mediated T cell activation. Nature 342,85-87. 15. Brmster, R. , Braun, R. , Avarbock, M. , and Palmiter, R. D. (1989) Targeted correction of a maJor hrstocompatibrlity class II Ea gene by DNA mtcromjected mto mouse eggs. Proc. Natl. Acad. Scz. USA 86, 7087-7091. 16. Schwartz, R. H. (1985) T-lymphocyte recognition of antigen m association with gene products of the MaJor Histocompatibihty Complex. Ann. Rev. Immunol.
Transgenesis Techniques: Principles and Protocols Methods in Molecular Biology Vol 18 by David Murphy, David A. Carter